Label-Free Aptasensor for Lysozyme Detection Using Electrochemical Impedance Spectroscopy
نویسندگان
چکیده
منابع مشابه
Label-Free Aptasensor for Lysozyme Detection Using Electrochemical Impedance Spectroscopy
This research develops a label-free aptamer biosensor (aptasensor) based on graphite-epoxy composite electrodes (GECs) for the detection of lysozyme protein using Electrochemical Impedance Spectroscopy (EIS) technique. The chosen immobilization technique was based on covalent bonding using carbodiimide chemistry; for this purpose, carboxylic moieties were first generated on the graphite by elec...
متن کاملDesigning a Label Free Aptasensor for Detection of Methamphetamine
A label-free electrochemical nucleic acid aptasensor for the detection of methamphetamine (MA) by the immobilization of thiolated self-assembled DNA sequences on a gold nanoparticles-chitosan modified electrode is constructed. When MA was complexed specifically to the aptamer, the configuration of the nucleic acid aptamer switched to a locked structure and the interface of the biosensor changed...
متن کاملdesigning a label free aptasensor for detection of methamphetamine
a label-free electrochemical nucleic acid aptasensor for the detection of methamphetamine (ma) by the immobilization of thiolated self-assembled dna sequences on a gold nanoparticles-chitosan modified electrode is constructed. when ma was complexed specifically to the aptamer, the configuration of the nucleic acid aptamer switched to a locked structure and the interface of the biosensor changed...
متن کاملLabel-free impedimetric aptasensor for lysozyme detection based on carbon nanotube-modified screen-printed electrodes.
We report on the direct electrochemical detection of aptamer-protein interactions, namely between a DNA aptamer and lysozyme (LYS) based on electrochemical impedance spectroscopy (EIS) technique. First, the affinity of the aptamer to LYS and control proteins was presented by using filter retention assay. An amino-modified version of the DNA aptamer-recognizing lysozyme was covalently immobilize...
متن کاملLabel-Free Toxin Detection by Means of Time-Resolved Electrochemical Impedance Spectroscopy
The real-time detection of trace concentrations of biological toxins requires significant improvement of the detection methods from those reported in the literature. To develop a highly sensitive and selective detection device it is necessary to determine the optimal measuring conditions for the electrochemical sensor in three domains: time, frequency and polarization potential. In this work we...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Proceedings
سال: 2017
ISSN: 2504-3900
DOI: 10.3390/proceedings1080734